Part:BBa_K2978101:Design

AgrB Clostridioides difficile gene

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 370
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

None

Source

Synthesize de novo with codon optimization for E. coli

References

Martin, M. J., Clare, S., Goulding, D., Faulds-Pain, A., Barquist, L., Browne, H. P., … Wren, B. W. (2013). The agr Locus Regulates Virulence and Colonization Genes in Clostridium difficile 027. Journal of Bacteriology, 195(16), 3672–3681. doi:10.1128/jb.00473-13.

Lubkowicz, D., Ho, C. L., Hwang, I. Y., Yew, W. S., Lee, Y. S., & Chang, M. W. (2018). Reprogramming Probiotic Lactobacillus reuteri as a Biosensor for Staphylococcus aureus Derived AIP-I Detection. ACS Synthetic Biology, 7(5), 1229–1237. doi:10.1021/acssynbio.8b00063.

Reynolds, J., & Wigneshweraraj, S. (2011). Molecular Insights into the Control of Transcription Initiation at the Staphylococcus aureus agr operon. Journal of Molecular Biology, 412(5), 862–881. doi:10.1016/j.jmb.2011.06.018.

Rajasree, K., Fasim, A., & Gopal, B. (2016). Conformational features of the Staphylococcus aureus AgrA-promoter interactions rationalize quorum-sensing triggered gene expression. Biochemistry and Biophysics Reports, 6, 124–134. doi:10.1016/j.bbrep.2016.03.012.